Amsterdam is one of the most bicycle-friendly large cities in the world and is a centre of bicycle culture with good facilities for cyclists such as bike paths and bike racks, and several guarded bike storage garages (Fietsenstalling) which can be used for a nominal fee. In 2006, there were about 465,000 bicycles in Amsterdam. Theft is widespread - in 2005, about 54,000 bicycles were stolen in Amsterdam. Bicycles are used by all socio-economic groups because of their convenience, Amsterdam's small size, the 400 km of bike paths, the flat terrain, and the arguable inconvenience of driving an automobile. The bike paths or (Fietspad) are coloured brown, in order to differentiate them from pedestrian sidewalks. A wide variety of bicycles are used, such as road bicycles, mountain bikes, racing bikes and even recumbent bikes, but the vast majority of bicycles are second-hand, older-model, heavy bikes with one gear and back-pedal coaster brakes. Many tourists discover Amsterdam by bike, which is the typical Dutch way. Bike Tour Amsterdam offers a guided bike tour through the city. Bicycle traffic, and traffic in general, is relatively safe - in 2007, Amsterdam had a total of 18 traffic deaths, compared with 26 people murdered.

In the city centre, driving a car is discouraged. Parking fees are expensive, and many streets are closed to cars or are one-way. The local government sponsors carsharing and carpooling initiatives such as Autodelen and Meerijden.nu.

Public transport in Amsterdam mainly consists of (night)bus and tram lines operated by Gemeentelijk Vervoerbedrijf. Regional buses, and some suburban buses, are operated by Connexxion and Arriva. Currently, there are 16 different tramlines. There are currently four metro lines, with a fifth line, the North/South line, under construction. Three free ferries carry pedestrians and cyclists across the IJ to Amsterdam-Noord, and two fare-charging ferries run east and west along the harbour. There are also water taxis, a water bus, a boat sharing operation, electric rental boats (Boaty) and canal cruises, that transport people along Amsterdam's waterways.

The A10 ringroad surrounding the city connects Amsterdam with the Dutch national network of freeways. Interchanges on the A10 allow cars to enter the city by transferring to one of the eighteen city roads, numbered S101 through to S118. These city roads are regional roads without grade separation, and sometimes without a central reservation. Most are accessible by cyclists. The S100 Centrumring is a smaller ringroad circumnavigating the city's centre.

Amsterdam was intended in 1932 to be the hub, a kind of Kilometre Zero, of the highway system of the Netherlands,with freeways numbered one through eight planned to originate from the city. The outbreak of the Second World War and shifting priorities led to the current situation, where only roads A1, A2, and A4 originate from Amsterdam according to the original plan. The A3 road to Rotterdam was cancelled in 1970 in order to conserve the Groene Hart. Road A8, leading north to Zaandam and the A10 Ringroad were opened between 1968 and 1974.
Besides the A1, A2, A4 and A8, several freeways, such as the A7 and A6, carry traffic mainly bound for Amsterdam.

Amsterdam is served by ten stations of the Nederlandse Spoorwegen (Dutch Railways). Five are intercity stops: Sloterdijk, Zuid, Amstel, Bijlmer ArenA and Amsterdam Centraal. The stations for local services are: Lelylaan, RAI, Holendrecht, Muiderpoort and Science Park. Amsterdam Centraal is also an international train station. From the station there are regular services to destinations such as Austria, Belarus, Belgium, the Czech Republic, Denmark, France, Germany, Hungary, Poland, Russia and Switzerland. Among these trains are international trains of the Nederlandse Spoorwegen and the Thalys(Amsterdam-Brussels-Paris-Cologne), CityNightLine, and InterCityExpress.

Eurolines has coaches from Amsterdam Amstel railway station to destinations all over Europe.

Amsterdam Airport Schiphol is less than 20 minutes by train from Amsterdam Central Station. It is the biggest airport in the Netherlands, the fifth largest in Europe, and the twelfth largest in the world in terms of passengers. It handles about 46 million passengers per year and is the home base of three airlines, KLM, transavia.com and Martinair. Schiphol was, in 2006, the third busiest airport in the world measured by international passengers.